Selective exo-enzymatic labeling of N-glycans on the surface of living cells by recombinant ST6Gal I
重组 ST6Gal I 对活细胞表面 N-糖链进行选择性酶外标记
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作者：Ngalle Eric Mbua, Xiuru Li, Heather R Flanagan-Steet, Lu Meng, Kazuhiro Aoki, Kelley W Moremen, Margreet A Wolfert, Richard Steet, Geert-Jan Boons
Abstract
A game of tag: N-Glycans on the surface of living cells were selectively tagged by exogenously administering recombinant ST6Gal I sialyltransferase and azide-modified CMP-Neu5Ac. This modification was followed by a strain-promoted cycloaddition using a biotin-modified dibenzylcyclooctynol (red star=biotin). The methodology will make it possible to dissect the mechanisms that underlie altered glycoconjugate recycling and storage in disease.
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