Conclusion
The gyrB PCR assay is now validated for easy identification of A. baumannii in comparison with gold standard WGS-based assays.
Methods
We cultured 270 sequential A. baumannii isolates from the rectal swabs of 32 adult patients. The identity of the isolates was determined by gyrB PCR. The sequences of 269 isolates were determined by Illumina sequencing and the taxonomy was inferred by the Kraken 2 program and ANI.
Objective
A multiplex gyrB PCR assay has been used to diagnose Acinetobacter baumannii. However, this assay has not been validated against the gold standard DNA-DNA hybridization assay, which is a laborious method. DNA-DNA hybridization assay is now replaced by whole genome sequence (WGS)-based
Results
All the 269 isolates were confirmed as A. baumannii by Kraken 2 and ANI.