Abstract
A Gram-stain-positive, rod-shaped bacterium, designated as HLT2-17(T), was isolated from soil sample taken from the Hailuogou glacier in Sichuan province, PR China. Strain HLT2-17(T) was capable of growing at 4-25°C and in NaCl concentrations ranging from 0 to 2% (w/v). The highest level of 16S rRNA gene sequence similarity was observed with Pengzhenrongella phosphoraccumulans M0-14(T) (98.3 %) and Pengzhenrongella sicca LRZ-2(T) (98.2 %). The average nucleotide identity and digital DNA-DNA hybridization values between strain HLT2-17(T) and its closest relatives, P. phosphoraccumulans M0-14(T) and P. sicca LRZ-2(T), were 80.0-84.0 % and 23.3-27.7 %, respectively. Phylogenomic analysis indicated that strain HLT2-17(T) clustered together with strains P. phosphoraccumulans M0-14(T) and P. sicca LRZ-2(T). Strain HLT2-17(T) contained C(16 : 0) and anteiso-C(15 : 0) as the major fatty acids, and MK-9(H(4)) as the menaquinone. Therefore, based on a polyphasic approach, we propose that strain HLT2-17(T) (=CGMCC 1.11116(T)= NBRC 110443(T)) represents a novel species of the genus Pengzhenrongella and suggest the name Pengzhenrongella frigida sp. nov.