Abstract
A bacterial strain, named For3(T), was isolated from forest soil sampled in Champenoux, France. Based on its 16S rRNA gene sequence, the strain was affiliated to the family Streptomycetaceae and, more specifically, to the genus Streptomyces. The strain had 99.93% 16S rRNA gene sequence similarity to its closest relative strains Streptomyces pratensis ATCC 33331(T), Streptomyces anulatus ATCC 27416(T), Streptomyces setonii NRRL ISP-5322(T) and Kitasatospora papulosa NRRL B-16504(T). The phylogenomic tree using the genome blast distance phylogeny method showed that the closest relative strain was Streptomyces atroolivaceus NRRL ISP-5137(T) and that For3(T) represents a new branch among the Streptomyces. Genome relatedness indexes revealed that the average nucleotide identity and digital DNA-DNA hybridization values between For3(T) and its closest phylogenomic relative (S. atroolivaceus NRRL ISP-5137(T)) were 88.39 and 39.2 %, respectively. The G+C content of the genome was 71.4 mol% and its size was 7.96 Mb with 7492 protein-coding genes. Strain For3(T) harboured complete metabolic pathways absent in the closest relative strains such as cellulose biosynthesis, glycogen degradation I, glucosylglycerate biosynthesis I. Anteiso-C(15:0), iso-C(15:0), anteiso-C(17:0) and MK-9(H4)/MK-9(H6) were the predominant cellular fatty acids and respiratory quinones, respectively. Phenotypic and genomic data supported the assignment of strain For3(T) to a novel species Streptomyces silvae sp. nov., within the genus Streptomyces, for which the type strain is For3(T) (=CIP 111908(T)=LMG 32186(T)).