A fast and efficient procedure to produce scFvs specific for large macromolecular complexes

一种快速高效制备针对大型大分子复合物的scFv的方法

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Abstract

We have expanded the application of antibody phage display to a new type of antigen: ribonucleoprotein (RNP) complexes. We describe a simple and efficient method for screening antibodies specific for large intact RNPs and individual components. We also describe a fast and easy method to overcome the abundance of amber stop codons in the positive phage clones. The resulting antibodies have been used in ELISA and Western blot analysis.

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