Comparison of DNA methylation patterns of parentally imprinted genes in placenta derived from IVF conceptions in two different culture media

两种不同培养基中 IVF 受孕胎盘亲本印迹基因 DNA 甲基化模式比较

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作者：Callista L Mulder, Tess M Wattimury, Aldo Jongejan, Cindy M de Winter-Korver, Saskia K M van Daalen, Robert B Struijk, Susanne C M Borgman, Yvonne Wurth, Dimitri Consten, Jannie van Echten-Arends, Sebastiaan Mastenbroek, John C M Dumoulin, Sjoerd Repping, Ans M M van Pelt, Aafke P A van Montfoort

期刊：

Human Reproduction

影响因子：

6.000

时间：

2020

起止号：

2020 Mar 27;35(3):516-528.

doi：

10.1093/humrep/deaa004

研究方向：

信号转导、表观遗传

信号通路：

DNA甲基化

Methods

To identify differential DNA methylation of imprinted genes, we opted for an amplicon-based sequencing strategy on an Illumina MiSeq sequencing platform. DNA was isolated and 34 DMRs associated with well-defined parentally imprinted genes were amplified in a two-step PCR before sequencing using MiSeq technology. Sequencing data were analyzed in a multivariate fashion to eliminate possible confounding effects. Main

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