Abstract
A common approach for biological assessment is to compare current observations of biota at a site to predictions of the biota that would occur if the site were in reference condition. To estimate these reference expectations, predictive bioassessment models use only data collected at reference sites and estimate relationships between biota and environmental variables that are unaffected by human activities (i.e., immutable variables). However, in some areas where human activities are pervasive, few reference sites are available. Here, we introduce a new approach for bioassessment, in which we first estimate relationships between widely available measurements of both immutable and human-influenced landscape variables and local environmental conditions (conductivity, dissolved total P, total suspended solids, percentage of sand and fines in the substrate, and dissolved organic C), and then we estimate the relationship between these local environmental variables and total macroinvertebrate richness. We then calculate the values of the local environmental variables under reference conditions by adjusting predictors that are strongly influenced by human activities to levels that are consistent with those observed at reference sites. Reference values of local environmental variables are then combined with the model for taxon richness to calculate taxon richness expected under reference conditions. Predictions of total taxon richness in validation data using the new approach are similar in accuracy and precision as predictions calculated using a traditional approach that focuses only on reference site data. The new approach complements existing bioassessment methods by highlighting types of sites in which estimates of reference expectations are uncertain and by improving our understanding of how instream stressors affect expected total taxon richness.