Abstract
N(6)-methyladenosine (m(6)A) is involved in most biological processes and actively participates in the regulation of reproduction. According to recent research, long non-coding RNAs (lncRNAs) and their m(6)A modifications are involved in reproductive diseases. In the present study, using m(6)A-modified RNA immunoprecipitation sequencing (m(6)A-seq), we established the m(6)A methylation transcription profiles in patients with recurrent implantation failure (RIF) for the first time. There were 1443 significantly upregulated m(6)A peaks and 425 significantly downregulated m(6)A peaks in RIF. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses revealed that genes associated with differentially methylated lncRNAs are involved in the p53 signalling pathway and amino acid metabolism. The competing endogenous RNA network revealed a regulatory relationship between lncRNAs, microRNAs and messenger RNAs. We verified the m(6)A methylation abundances of lncRNAs by using m(6)A-RNA immunoprecipitation (MeRIP)-real-time polymerase chain reaction. This study lays a foundation for further exploration of the potential role of m(6)A modification in the pathogenesis of RIF.