Abstract
The dynamics of the resting-state activity in brain functional networks are complex, containing meaningful patterns over multiple temporal scales. Such physiologic complexity is often diminished in older adults. Here we aim to examine if the resting-state complexity within functional brain networks is sensitive to functional status in older adults and if repeated exposure to transcranial direct current stimulation (tDCS) would modulate such complexity. Twelve older adults with slow gait and mild-to-moderate executive dysfunction and 12 age- and sex-matched controls completed a baseline resting-state fMRI (rs-fMRI). Ten participants in the functionally-limited group then completed ten 20-minute sessions of real (n = 6) or sham (n = 4) tDCS targeting the left prefrontal cortex over a two-week period as well as a follow-up rs-fMRI. The resting-state complexity associated with seven functional networks was quantified by averaging the multiscale entropy (MSE) of the blood oxygen level-dependent (BOLD) time-series for all voxels within each network. Compared to controls, functionally-limited group exhibited lower complexity in the motor, ventral attention, limbic, executive and default mode networks (F > 6.3, p < 0.02). Within this group, those who received tDCS exhibited greater complexity within the ventral, executive and limbic networks (p < 0.04) post intervention as compared to baseline, while no significant changes in sham group was observed. This study provides preliminary evidence that older adults with functional limitations had diminished complexity of resting-state brain network activity and repeated exposure to tDCS may increase that resting-state complexity, warranting future studies to establish such complexity as a marker of brain health in older adults.