Real-time voltammetric detection of cocaine-induced dopamine changes in the striatum of freely moving mice

利用实时伏安法检测自由活动小鼠纹状体中可卡因诱导的多巴胺变化

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Abstract

In the present voltammetric study, we have characterized cocaine-induced changes in evoked dopamine release and uptake in the striatum of freely moving mice in real time. Cocaine induced marked dopamine uptake inhibition measured as apparent K(m) changes, producing a maximal effect 20min following a single injection (15mg/kg, i.p.). Changes in uptake were paralleled by increases in evoked dopamine release per stimulus pulse, revealing a high correlation between these two parameters following cocaine administration. This initial characterization of cocaine effects on striatal dopamine transmission in the commonly used C57BL/6 mouse strain provides a basis for future voltammetric studies using genetic mouse models.

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