Abstract
While there is no conclusive evidence that monosodium glutamate (MSG, a food additive) directly causes liver cancer in humans, certain studies suggest a potential link between MSG-induced liver injury and cancer development. This study aimed to evaluate the protective effect of tannic acid (TA, a natural polyphenol) against MSG-induced hepatotoxicity through the glutathione and thioredoxin systems. Twenty-four rats were randomly divided into control and experimental groups and treated with TA, MSG, and MSG+TA once daily by oral gavage for 21 days. In addition to major oxidative stress indicators (total glutathione; GSH + GSSG and malondialdehyde; MDA), mRNA expression changes and biological activity responses of components of the glutathione and thioredoxin systems were examined in the liver tissues of all animals. The results showed that MSG alone negatively affected both stress indicators and antioxidant system components (glutathione peroxidase; GPx, glutathione reductase; GR, glutathione-S-transferase; GST, and thioredoxin reductase; TrxR) in terms of mRNA expression and biological activity. However, the combination of MSG and TA demonstrated robust antioxidative effects, surpassing the outcomes of MSG treatment. Our results provide new insights into pivotal molecular targets and protective candidates that should be focused on in future in vivo and in vitro HCC research.