Abstract
The complete chloroplast genome sequence of Haplopteris flexuosa, a member of Vittarioideae (family Pteridaceae), was determined. The chloroplast genome of H. flexuosa was 165,664 bp in length with two inverted repeats (32,556 bp) between a large single copy (79,996 bp) and a small single copy (20,556 bp). The GC content was higher than that of related taxa H. elongate, and it was caused by high GC content of expanded regions by insertion of mobile open reading frames in fern organelles (MORFFO) found in the family Pteridaceae. And also, we found that rrn5-rps12 enlarged region of H. flexuosa was similar to MORFFOs of other Pteridaceae.