Abstract
BACKGROUND: Infectious diseases are increasingly recognized as public health concern worldwide as the rising incidence in multidrug resistance bacteria. This consequently enforces the need to find a new antimicrobial agent where plants have a potential source. This study investigated the antibacterial activity of fruit pulp extract of the Tamarindus indica against Staphylococcus aureus (S. aureus) and Klebsiella pneumoniae (K. pneumoniae). METHODS AND MATERIALS: Maceration technique was employed for subsequent extraction of the sample using acetone and ethanol. Antibacterial activity of the plant extract was investigated based on minimum inhibitory concentration (MIC) against Gram-negative strain (K. pneumoniae (ATCC 700603)) and Gram-positive strain (S. aureus (ATCC 25923)) using agar disc-diffusion technique. RESULTS: It was found that both acetone and ethanol extracts showed significant antibacterial activities, against both S. aureus and K. pneumoniae as compared to the negative control (P = 0.00), but no significantly different from the drug (P > 0.05). However, K. pneumoniae showed more sensitivity to the extracts than S. aureus with MIC value of 18.75 mg/mL and 9.38 mg/mL for both acetone and ethanol extracts against S. aureus and K. pneumoniae respectively. CONCLUSION: This study suggested that the fruit pulp have antibacterial properties, which might validate their traditional uses.