5'-deoxy-5'-hydrazinylguanosine as an initiator of T7 Rna polymerase-catalyzed transcriptions for the preparation of labeling-ready RNAs
5'-脱氧-5'-肼基鸟苷作为 T7 Rna 聚合酶催化转录的启动子，用于制备可标记的 RNA
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作者：Mark Skipsey, Gordon Hack, Thomas A Hooper, Mark C Shankey, Louis P Conway, Martin Schröder, David R W Hodgson
Abstract
5'-deoxy-5'-hydrazinylguanosine was incorporated into the 5'-termini of RNA transcripts using T7 RNA polymerase. Transcriptions provided 5'-hydrazinyl-RNA that was readily labeled and purified. The use of fluorophore-labeled material was validated in an endoribonuclease activity assay.
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