The best of both worlds: Combining lineage-specific and universal bait sets in target-enrichment hybridization reactions

两全其美:在靶向富集杂交反应中结合谱系特异性和通用诱饵组

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作者:Kasper P Hendriks, Terezie Mandáková, Nikolai M Hay, Elfy Ly, Alex Hooft van Huysduynen, Rubin Tamrakar, Shawn K Thomas, Oscar Toro-Núñez, J Chris Pires, Lachezar A Nikolov, Marcus A Koch, Michael D Windham, Martin A Lysak, Félix Forest, Klaus Mummenhoff, William J Baker, Frederic Lens, C Donovan Ba

Conclusions

High levels of enrichment and locus reconstruction for the two target sets demonstrate that the sampling of genomic regions can be easily extended through the combination of probe sets in single enrichment reactions. We hope that these findings will facilitate the production of expanded data sets that answer individual research questions and simultaneously allow wider applications by the research community as a whole.

Results

Using 26 Brassicaceae libraries and standard enrichment protocols, we compare results from three independent data sets. A large average fraction of reads mapping to the Angiosperms353 (24-31%) and Brassicaceae (35-59%) targets resulted in a sizable reconstruction of loci for each target set (x̄ ≥ 70%). Conclusions: High levels of enrichment and locus reconstruction for the two target sets demonstrate that the sampling of genomic regions can be easily extended through the combination of probe sets in single enrichment reactions. We hope that these findings will facilitate the production of expanded data sets that answer individual research questions and simultaneously allow wider applications by the research community as a whole.

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