Abstract
INTRODUCTION: Accurate intraoperative identification of glioma molecular subtypes, such as isocitrate dehydrogenase mutation and 1p/19q co-deletion, is essential for precise diagnosis, prognostication, and determining the extent of tumor resection-balancing maximal tumor removal with preservation of neurological function. METHODS: We developed a machine learning model that integrates preoperative imaging features [magnetic resonance imaging, computed tomography, and (11)C-methionine positron emission tomography (PET)] and intraoperative flow cytometry (iFC) data to predict molecular subtypes of glioma in real-time. RESULTS: Analyzing 288 cases of diffuse gliomas, this model achieved an overall accuracy of 76.0%, with a macro-average ROC-AUC of 0.88 and a micro-average ROC-AUC of 0.89. Key predictive factors included the tumor-to-normal uptake ratio on PET, malignancy index from iFC, and patient age, all of which showed significant differences between correctly and incorrectly classified cases. We also developed a prototype application that visualizes the prediction results intraoperatively, thereby supporting real-time surgical decision-making. CONCLUSION: This integrated approach enhances the precision of intraoperative molecular diagnosis and has the potential to optimize surgical strategies for glioma treatment.