Abstract
Microplitis varicolor (Hymenoptera: Braconidae) is a parasitoid wasp species with potential for biological control of lepidopteran pests. In this study, the complete mitochondrial genome of M. varicolor was sequenced and characterized. The mitogenome is 15,580 bp in length and exhibits a strong AT bias (86.82%). It contains 37 mitochondrial genes, including 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and a control region. Phylogenetic analyses support the monophyly of Braconidae and place M. varicolor firmly within the genus Microplitis. The genome exhibits largely conserved organization with several tRNA rearrangements, providing a valuable resource for phylogenetic and evolutionary studies of Braconidae.