Crystallization and preliminary X-ray diffraction analysis of a protease-resistant mutant form of human galectin-8

人半乳糖凝集素-8蛋白酶抗性突变体的结晶和初步X射线衍射分析

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Abstract

A crystal of a protease-resistant mutant form of human galectin-8, a tandem-repeat-type galectin with two carbohydrate-recognition domains, was obtained using the hanging-drop method and was found to belong to the tetragonal space group P4(3)2(1)2, with unit-cell parameters a = 78.93, b = 78.93, c = 132.05 A. Diffraction data were collected to a resolution of 3.4 A.

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