Crystallization and preliminary X-ray analysis of PH1010 from Pyrococcus horikoshii OT3, a member of the archaeal DUF54 family of proteins

PH1010 from Pyrococcus horikoshii OT3, a member of the archaeal DUF54 family of proteins, was expressed, purified and crystallized. Crystallization was performed by the sitting-drop vapour-diffusion method using PEG 3350 as the precipitant. The crystal diffracted X-rays to 1.90 A resolution using a synchrotron-radiation source. The space group of the crystal was determined to be P2(1)2(1)2(1), with unit-cell parameters a = 46.9, b = 49.5, c = 132.7 A. The crystal contained two PH1010 molecules in the asymmetric unit (V(M) = 2.4 A(3) Da(-1)) and had a solvent content of 48%.