Abstract
OBJECTIVE: ApoC-III (apolipoprotein C-III) glycosylation can predict cardiovascular disease risk. Higher abundance of disialylated (apoC-III(2)) over monosialylated (apoC-III(1)) glycoforms is associated with lower plasma triglyceride levels. Yet, it remains unclear whether apoC-III glycosylation impacts TRL (triglyceride-rich lipoprotein) clearance and whether apoC-III antisense therapy (volanesorsen) affects distribution of apoC-III glycoforms. Approach and Results: To measure the abundance of human apoC-III glycoforms in plasma over time, human TRLs were injected into wild-type mice and mice lacking hepatic TRL clearance receptors, namely HSPGs (heparan sulfate proteoglycans) or both LDLR (low-density lipoprotein receptor) and LRP1 (LDLR-related protein 1). ApoC-III was more rapidly cleared in the absence of HSPG (t(1/2)=25.4 minutes) than in wild-type animals (t(1/2)=55.1 minutes). In contrast, deficiency of LDLR and LRP1 (t(1/2)=56.1 minutes) did not affect clearance of apoC-III. After injection, a significant increase in the relative abundance of apoC-III(2) was observed in HSPG-deficient mice, whereas the opposite was observed in mice lacking LDLR and LRP1. In patients, abundance of plasma apoC-III glycoforms was assessed after placebo or volanesorsen administration. Volanesorsen treatment correlated with a statistically significant 1.4-fold increase in the relative abundance of apoC-III(2) and a 15% decrease in that of apoC-III(1). The decrease in relative apoC-III(1) abundance was strongly correlated with decreased plasma triglyceride levels in patients. CONCLUSIONS: Our results indicate that HSPGs preferentially clear apoC-III(2). In contrast, apoC-III(1) is more effectively cleared by LDLR/LRP1. Clinically, the increase in the apoC-III(2)/apoC-III(1) ratio on antisense lowering of apoC-III might reflect faster clearance of apoC-III(1) because this metabolic shift associates with improved triglyceride levels.