Abstract
Human monocyte subsets have traditionally been defined based on expression of CD14 and CD16 into classical, non-classical and intermediate monocytes. Using CyTOF, Thomas et.al. identified CCR2, CD36, HLA-DR and CD11c as additional cell surface markers that provide better resolution of intermediate and non-classical monocyte subsets. These markers can be used in traditional flow cytometry to identify alterations in subset frequencies during clinical studies.