Lead Acetate Exposure and Cerebral Amyloid Accumulation: Mechanistic Evaluations in APP/PS1 Mice

The role of environmental factors in Alzheimer's disease (AD) pathogenesis remains elusive. Mounting evidence suggests that acute and past exposure to the environmental toxicant lead (Pb) is associated with longitudinal decline in cognitive function, brain atrophy, and greater brain ββ<math><mi>β</mi></math>-amyloid (AβAβ<math><mrow><mi>A</mi><mi>β</mi></mrow></math>) deposition. However, the nature of Pb-induced amyloid deposition and how it contributes to AD development remain unclear. Objectives: This study investigates the role of Pb in the pathogenesis of cerebral amyloid angiopathy (CAA) and whether plasminogen activator inhibitor-1 (PAI-1) contributes to this process in the APP/PS1 mouse model.

Our findings suggest that PbAc induced CAA/AD pathogenesis via the PAI-1 signaling in the APP/PS1 mouse model, and the inhibition of PAI-1 could be a potential therapeutic target for PbAc-mediated CAA/AD disorders. https://doi.org/10.1289/EHP14384.

Female APP/PS1 mice at 8 wk of age were administered either 50mg/kg50mg/kg<math><mrow><mn>50</mn><mspace></mspace><mi>mg</mi><mo>/</mo><mi>kg</mi></mrow></math> Pb-acetate (PbAc) (i.e., 27mgPb/kg27mgPb/kg<math><mrow><mn>27</mn><mspace></mspace><mi>mg</mi><mtext> </mtext><mi>Pb</mi><mo>/</mo><mi>kg</mi></mrow></math>) or an equivalent molar concentration of sodium acetate (NaAc) via oral gavage once daily for 8 wk. Amyloid deposition and vascular amyloid were determined by immunostaining. In addition, AβAβ<math><mrow><mi>A</mi><mi>β</mi></mrow></math> perivascular drainage, vascular binding assay, and microglial endocytosis were examined to determine underlying mechanisms. Furthermore, magnetic resonance imaging demyelination imaging was performed in vivo measure the level of demyelination. Finally, Y-maze and Morris water maze tests were assessed to evaluate the cognitive function of mice.

APP/PS1 mice (an AD mice model) exposed to PbAc demonstrated more vascular amyloid deposition less neocortical myelination, and lower cognitive function, as well as greater vascular binding to Aβ40Aβ40<math><mrow><mi>A</mi><mi>β</mi><mn>40</mn></mrow></math>, higher Aβ40/Aβ42Aβ40/Aβ42<math><mrow><mi>A</mi><mi>β</mi><mn>40</mn><mo>/</mo><mi>A</mi><mi>β</mi><mn>42</mn></mrow></math> ratios, strikingly lower Aβ40Aβ40<math><mrow><mi>A</mi><mi>β</mi><mn>40</mn></mrow></math> levels in the perivascular drainage, and microglial endocytosis. Importantly, exposure to a specific PAI-1 inhibitor, tiplaxtinin, which previously was reported to lower CAA pathology in mice, resulted in less CAA-related outcomes following PbAc exposure.