Abstract
Proteins are the functional constituents of cells. The diverse physical properties of proteins enable them to be multifunctional and also pose challenges to analyzing their abundance, subcellular localization, and potential interacting partners using proteomics. Some samples are especially recalcitrant and pose additional challenges in proteomic analysis due to the unanticipated modification of proteins. These challenges extend from initial protein extraction to analysis using mass spectrometry. Broadly, the focus of workshop will be to discuss the benefits and limitations of using proteomics to study protein identification, protein quantification, protein structure, and proteinprotein interactions. Examples will be drawn from different biological disciplines, including cell biology, host-pathogen biology, and developmental biology to demonstrate how scientists are solving the problems posed by their discovery methods and biological systems. The workshop will include presentations from each panelist followed by an open discussion and question and answer session.