Abstract
Mammalian splenic tissue is rich in functional immune cells, primarily lymphocytes which can mask low-abundance populations in downstream analyses. This protocol enriches minority immune cell populations from mouse spleen via immunomagnetic negative depletion to generate an untouched enriched cell fraction. Enriched cells are then spiked with untouched splenocytes in a controlled repopulation, validated by flow cytometry and results in a single-cell transcriptomic clustering analysis with a broadened cellular landscape.
Keywords:
Antibody; Bioinformatics; Cell Biology; Cell isolation; Cell separation/fractionation; Flow Cytometry/Mass Cytometry; Gene Expression; Immunology; Model Organisms; Molecular Biology; RNAseq; Sequence analysis; Sequencing; Single Cell.