Abstract
The auto-combustion method synthesized CuO NPs and Ag/CuO NPs. The Ag/CuO NPs were analyzed using Fourier-transform infrared, X-ray diffraction, scanning electron microscope, and Energy-dispersive X-ray spectroscopy instrumental analyses. The energy band gap, as determined by DRS properties, decreases from 3.82 to 3.50 eV for pure CuO and 10% Ag/CuO NPs, respectively. The photodegradation efficiency of Rhodamine-B & Carmine by 10% Ag/CuO NPs was nearly 98.9 and 97.8%, respectively. Antimicrobial trials revealed that the antimicrobial efficacy of Ag/CuO NPs at several dosages (20, 40, 60, 80, 100, and 120 µg/mL) against human pathogens was initially assessed using the agar well-diffusion method, and then the broth dilution method. Noticeably, the minimum inhibitory concentration of Ag/CuO NPs for all pathogens ranged from 100 to 120 µg/ml, was determined. Generally, the observed minimum microbicide concentration has a wide range of Ag/CuO NPs doses, ranging from 150 to 300 µg/ml, which helps kill (99.99%) all tested pathogenic cells. The largest relative inhibitory activities (%) were recorded against Escherichia coli (81.45 ± 1.39) at 120 g/mL of Ag/CuO NPs and 100 μg/mL (80.43 ± 0.59), followed by 80 µg/mL (72.33 ± 0.82). Additionally, the lowest relative inhibitory activities (%) were monitored versus fungal cells and Gram-positive bacteria at 120 µg/mL of Ag/CuO NPs as 52.17 ± 1.49 and 53.42 ± 1.71; respectively.