Abstract
Grazing livestock in grasslands face the challenge of obtaining sufficient nutrition due to uneven distribution of plant species and fluctuating vegetation productivity and nutrient levels. In northern China, Leymus chinensis and Stipa grandis are the dominant perennial species in native grasslands, but they provide limited nutrition compared to forbs with higher crude protein (CP) content. While dietary ingredients can affect the nutritional intake of grazing livestock, the influence of different grazing strategies on dietary selection remains unclear. In this study, three sheep grazing strategies (lambs alone, mixed lambs and ewes, and ewes alone) were used to explore dietary selection. We investigated the influence of vegetation characteristic (above-ground biomass production, height, and species diversity) and foraging behavior (feed intake, organic matter digestibility, and daily grazing time) on the mechanisms of dietary selection (taxonomic family richness and composition). Forage consumption across the grazing strategies revealed that species from Poaceae, Rosaceae, and Cyperaceae families were frequently consumed. Both ewes and lambs in the mixed-grazing strategy preferentially consumed forbs with diverse species composition (Jacob's D > 0), which contained higher CP than those available in the overall vegetation (p < 0.05). In addition, dietary richness was significantly (p < 0.05) influenced by vegetation species diversity except for animals in the lambs alone strategy. Compared to lambs in mixed-grazing, lambs alone had both greater daily grazing time and consumption of grass with lower digestibility (p < 0.05). Our study is the first to demonstrate that lambs can develop a similar dietary selection and behavioral pattern when grazing with adult ewes in temperate grasslands. Our study indicates that the conservation of species diversity in native grasslands is critically beneficial to livestock nutrition.