Abstract
The complete mitochondrial genome of Rasbora atridorsalis Kottelat and Chu (Spixiana. 10(3):313-318) is first characterized herein (GenBank accession: OM617726.1). The circular mitogenome is 16,524 base pairs (bp) in length, containing 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and two non-coding regions, with an AT bias of 58.7%. A Bayesian phylogenetic reconstruction, based on the amino acid sequences of 13 mitochondrial protein-coding genes from R. atridorsalis and 41 congeneric species, resolved the evolutionary relationships within the genus, providing molecular evidence for intra-generic affinities. This work fills a critical genomic gap in Rasbora phylogenetics, provides a molecular basis for taxonomic revisions, and enhances understanding of the evolutionary history and interspecific relationships in Rasborinae.