Abstract
PURPOSE: Diabetic macular edema (DME) is a major cause of vision loss in patients with diabetic retinopathy; this study is aimed at comparing the expression of aquaporins (AQPs) on the inner limiting membranes (ILMs) of various vitreoretinal diseases and investigating the role of aquaporins expressed on the ILMs in mediating the occurrence of DME. METHODS: The whole-mounted ILM specimens surgically excised from patients with various vitreoretinal diseases (idiopathic macular hole, myopic traction maculopathy, and diabetic retinopathy) were analyzed by immunohistochemistry (IHC). The distribution and morphology of AQP4, AQP7, and AQP11 on the ILMs were correlated with immunohistochemical staining characteristics. Moreover, immunofluorescence of AQP4 was performed on the ILM specimens of the patient in four groups: the control group, negative control group, no DME group, and DME group. The immunofluorescence intensity value of AQP4 was measured using ImageJ. The difference between the four groups and the correction between the immunofluorescence value and central foveal thickness (CFT) were analyzed. RESULTS: In IHC sections, the expression of AQP4, AQP7, and AQP11 on ILMs of diabetic retinopathy (DR) with macular edema, respectively, seemed to be more abundant than in the idiopathic macular hole (iMH) and myopic traction maculopathy (MTM). Moreover, markedly higher fluorescence intensity of AQP4 of ILMs was determined in the DME group (51.05 ± 5.67) versus the other three groups (P < 0.001). A marked positive association was identified between the fluorescence intensity of AQP4 and CFT (r = 0.758; P = 0.011). CONCLUSIONS: AQP4, AQP7, and AQP11 can be expressed on human ILM in vivo. The increased expression of AQPs on the ILMs of DR may be associated with the occurrence of DME. Moreover, the degree of DME may be positively correlated with the expression of AQP4 on the ILMs.