Objective
To explore the role of CTHRC1 in regulating the proliferation and apoptosis of papillary thyroid cancer cells. Objective: Papillary thyroid cancer TPC-1 cells were transfected with a small interfering RNA (siRNA) targeting CTHRC1, with the cells transfected with a scrambled sequence as the negative control. The changes in cell proliferation and apoptosis were assessed using cell counting kit-8 (CCK-8) and flow cytometry with AV/PI double staining, respectively. The expression of c-caspase-3, c-PARP1 and phosphorylation of ERK1/2 in the cells were examined with Western blotting. Objective: Transfection with the siRNA sequence significantly decreased the mRNA and protein levels of CTHRC1 in TCP-1 cells (P < 0.05). Compared with blank and negative control cells, TCP-1 cells with RNA interference of CTHRC1 showed significantly lowered proliferative activity and enhanced cell apoptosis (P < 0.05) with significantly increased expressions of c-caspase-3 and c-PARP1 and phosphorylation of ERK1/2 (P < 0.05). Objective: RNA interference of CTHRC1 promotes the proliferation and inhibits apoptosis of papillary thyroid cancer cells possibly by activating the ERK1/2 pathway. 目的: 探讨胶原三股螺旋重复蛋白1（CTHRC1）甲状腺乳头状癌TPC-1细胞增殖、凋亡的影响。 方法: 成功筛选干扰甲状腺乳头状癌TPC-1细胞CTHRC1表达的siRNA，以脂质体转染法将siRNA转染甲状腺乳头状癌TPC-1细胞，采用real-time PCR和Western blot检测转染后TCP-1细胞中CTHRC1mRNA和蛋白表达的变化。实验分组：将筛选的干扰序列转染TPC-1细胞作为干扰组（si-CTHRC1组），将随机阴性对照序列转染的TPC-1细胞作为阴性对照组（si-NC组），以不做任何处理TPC-1细胞为空白组（WT组）。采用CCK-8实验检测细胞增殖；流式细胞术AV/PI双染观察各组TCP-1细胞凋亡的变化；采用Western blot法检测各组TPC-1细胞中含剪切型半胱氨酸的天冬氨酸蛋白水解酶-3（c-Caspase-3）蛋白、剪切型多聚ADP核糖聚合酶1（c-PARP1）蛋白表达水平以及磷酸化细胞外信号调节激酶1/2(ERK1/2)表达水平。 结果: 将筛选出明显抑制TPC-1细胞CTHCR1表达的siRNA转染细胞后，TPC-1细胞中CTHRC1的mRNA和蛋白表达水平降低（P < 0.05）。与WT组和si-NC组相比，si-CTHRC1组的细胞活降低（P < 0.05）；与对照组相比，si-CTHRC1组的TPC-1细胞细胞凋亡率升高，Western blot实验发现：si-CTHRC1组的c-caspase-3、c-PARP1蛋白表达水平均升高（P < 0.05）；si-CTHRC1组ERK1/2蛋白的磷酸化水平增加。 结论: 干扰CTHRC1能够抑制TPC-1细胞的增殖并诱导凋亡，此过程可能通过激活ERK1/2信号通路介导。