Combination of an unbiased amplification method and a resequencing microarray for detecting and genotyping equine arteritis virus

结合无偏扩增方法和重测序微阵列技术检测和分型马动脉炎病毒

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Abstract

This study shows that an unbiased amplification method applied to equine arteritis virus RNA significantly improves the sensitivity of the real-time reverse transcription-quantitative PCR (RT-qPCR) recommended by the World Organization for Animal Health. Twelve viral RNAs amplified using this method were hybridized on a high-density resequencing microarray for effective viral characterization.

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