Sampling and decontamination method for culture of nontuberculous mycobacteria in respiratory samples of cystic fibrosis patients

囊性纤维化患者呼吸道样本中非结核分枝杆菌培养的采样和去污方法

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Abstract

We confirmed that chlorhexidine decontamination yielded more nontuberculous mycobacteria than did the N-acetyl-l-cysteine-NaOH-oxalic acid procedure from respiratory samples of cystic fibrosis patients on solid cultures. However, this improved recovery is mostly balanced if the latter is combined with liquid culture. Furthermore, none of the 145 cough swabs, used to sample young children, cultured positive, suggesting that swabs are low-quality samples.

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