Simple disk-based method for detection of Klebsiella pneumoniae carbapenemase-type beta-lactamase by use of a boronic acid compound

利用硼酸化合物检测肺炎克雷伯菌碳青霉烯酶型β-内酰胺酶的简便圆盘法

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Abstract

A disk potentiation method using carbapenems as substrates and 3-aminophenyl boronic acid as an inhibitor was evaluated for the detection of Klebsiella pneumoniae carbapenemase (KPC)-type beta-lactamases. When combined with nonsusceptibility to ertapenem, the method was easy to perform and reliably differentiated isolates producing KPC-type beta-lactamases from those producing other types of beta-lactamases.

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