Sulfolobus tokodaii ST0053 produces a novel thermostable, NAD-dependent medium-chain alcohol dehydrogenase
Sulfolobus tokodaii ST0053 产生一种新型的耐热、NAD 依赖的中链醇脱氢酶
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作者：Hisaaki Yanai, Katsumi Doi, Toshihisa Ohshima
Abstract
An alcohol dehydrogenase (ADH) gene, ST0053, from Sulfolobus tokodaii was expressed in Escherichia coli. The purified recombinant enzyme was an NAD-dependent medium-chain ADH with high thermostability and tolerance of a wide range of pHs. This is the first step in creating an experimental functionality library of 10 genes annotated as ADHs in the S. tokodaii genome.
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