Application of a subcellular membrane-antibody binding assay for the analysis of antigen expression in human tumours

应用亚细胞膜抗体结合试验分析人类肿瘤中的抗原表达

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Abstract

A micro-radioisotopic antiglobulin assay has been employed to determine the binding of monoclonal antibodies to subcellular membranes adsorbed to the wells of Terasaki Microtest Plates. A panel of 15 monoclonal antibodies was tested against 22 membrane preparations isolated from normal and malignant colo-rectal, breast and lung tissues. The assay provided an objective evaluation of antibody reactivity permitting the expression of major antigens in samples from individual surgical specimens to be defined.

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