Vanillin biosynthesis from sucrose ex-sugarcane: authentication of an alternative vanillin source through stable isotope data analysis

蔗糖和甘蔗中香兰素的生物合成:通过稳定同位素数据分析验证替代香兰素来源

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作者:Chad A Hansen

Abstract

As one of the largest volume flavor ingredients, vanillin remains an attractive target for development of a cost-effective and sustainable process to manufacture. Presented here is newly available data on the production of vanillin via fermentation in an engineered strain of Saccharomyces cerevisiae grown on sucrose ex-sugarcane. The use of the C4 plant source of carbohydrate resulted in a δ13C mean stable isotope ratio of -14.43 ‰ (SD = 0.24) relative to the V-PDB standard and a δ2H mean stable isotope ratio of -122.8 ‰ (SD = 2.9) relative to the SMOW standard by IRMS. The abundance of 14C in the fermentation derived vanillin averaged 14.01 dpm/gC (SD = 0.09) by AMS measurement. These data are compared to historical data collected on vanillin derived from a number of sources to provide a more holistic view on vanillin bulk isotope data based on its method of manufacture.

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