Abstract
This article summarizes the current knowledge regarding omics approaches, which include genomics, transcriptomics, proteomics and metabolomics, in the context of bio-hydrogen production in Chlamydomonas reinhardtii. In this paper, critical genes (HydA1, Hyd A2, Sulp, Tla1, Sta7, PFL1) involved in H(2) metabolism were identified and analyzed for their function in H(2) accumulation. Furthermore, the advantages of gene microarrays and RNA-seq were compared, as well as their applications in transcriptomic analysis of H(2) production. Moreover, as a useful tool, proteomic analysis could identify different proteins that participate in H(2) metabolism. This review provides fundamental theory and an experimental basis for H(2) production, and further research effort is needed in this field.