Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
常规PCR和实时PCR对虾WSD诊断的比较分析
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作者：C A G Leal, G A Carvalho-Castro, A C Cottorello, R C Leite, H C P Figueiredo
Abstract
The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples.
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