Abstract
Lipid bilayer membranes are a central structural feature of living cells, providing a wide range of functions including partitioning of organelles, mediating cell interaction with the environment, and modulating intracellular signaling processes. By capturing the fluidity of the natural membranes in a reductionist in vitro model, substrate supported lipid bilayers have emerged as a compelling model system for these structures. Furthermore, the ability to control the composition and mobility of this system at micro- and nanoscales inspired several new routes of biological and biotechnological investigation. Here, we describe key methods used to create multicomponent lipid bilayers, discuss design considerations important to making these systems, and demonstrate this process in the specific context of understanding juxtacrine cell signaling. Different fabrication techniques were combined to first pattern a surface with barriers to lipid diffusion and then spatially control the exposure of this surface to lipid vesicles, leading to local formation of bilayers of different composition. This multicomponent system was used as a platform for to mimic the natural organization of T cells and antigen presenting cells by presenting ligands to the T cell receptor and lymphocyte function-associated antigen-1 that are tethered to separate, closely juxtaposed regions of bilayer. Other technologies like using photochemical polymerization of lipids to pattern bilayers have also been discussed. The information gathered from evaluating membrane interactions in patterned lipid bilayers may lead to the development of membrane-based biomedical devices for conducting novel cell-based assays and potentially high-throughput drug screens targeting membranes or membrane-associated components.