A genome-wide identification and expression analysis of the class III peroxidase gene family in Mangifera indica under abiotic stresses and the MiPRX27 gene regulates oxidative stress

Class III peroxidases (PRXs) are plant-specific enzymes that play vital roles in various physiological processes. However, the functional roles of mango PRXs under stress conditions remain poorly understood. In this study, we identified 76 MiPRX genes, which are unevenly distributed across the mango chromosomes. RT-qPCR analysis revealed differential expression of most MiPRX genes under oxidative, drought, and salt stress conditions, with MiPRX27 showing a particularly prominent role. Under oxidative stress, heterologous overexpression of MiPRX27 in Arabidopsis enhanced lateral root formation and accelerated root growth, suggesting that MiPRX27 contributes to reducing plant sensitivity to oxidative stress. Overall, this study provides a theoretical foundation for further exploration of MiPRX-mediated mechanisms underlying mango stress tolerance.