Abstract
A gram-positive, nonpathogenic, central endospore-forming, flagellated strain, was successfully isolated from the rhizosphere of Pinus pinaster in Aracena (Spain). Its optimal growth conditions are 28 °C, pH 6, and 0 % salinity. It is able to assimilate glucose, L-fucose, L-arabinose, b-metil-D-xylose and shows high catabolic capacity. The major fatty acids (>79.20 % of the total fatty acids) are anteiso C15:0 > iso C15:0 > C14:0. A phylogenetic analysis based on the 16S rRNA gene sequence revealed similarity to P. frigoritolerans DSM8801(T) (99.9 %), P. castrilensis CECT30509(T) (99.8 %), and P. simplex DSM1321(T) (99.6 %). Comparison of whole genomes revealed that strain BBB004(T) is more similar to P. simplex DSM1321(T). According to ANI (93.54 %), AAI (94 %), dDDH (60.6), %G + C (0.12), TETRA (0.99822) and intergenomic distance (0.2835) values, therefore this species is different to the closest. A total of 133 genes unique to Peribacillus BBB004(T) were identified. Supported by these analyses, strain BBB004(T) (=LMG32742(T) = CCUG76477(T)) was proposed as the type strain for a new species, named "Peribacillus aracenensis sp. nov."