Abstract
OBJECTIVES: Thermophilic bacteria are valuable biotechnological resources. A thermophilic bacterium isolated from the Thai hot spring sediments was subjected to complete genome sequencing. Genome data will facilitate bacterial identification and comparative genomics within the Hydrogenophilus genus and provide insights into polyhydroxybutyrate biosynthesis pathways for industrial applications. DATA DESCRIPTION: The H. thermoluteolus KS0102 genome comprises a single circular chromosome containing 2,136,028 bp and 61.70% GC. The genome was sequenced using Illumina and Oxford Nanopore technologies and assembled using Unicycler. It contained 1,848 CDS, 9 rRNAs, 49 tRNAs, and 1 CRISPR region. This dataset enables the investigation of polyhydroxybutyrate biosynthesis pathways and comparative genomic studies of thermophilic adaptation mechanisms in Hydrogenophilus species.