Abstract
Chelidonium majus is a herbaceous plant of significant medicinal value, which has been widely distributed in Europe, Asia, and Northern Africa. However, its genome remains uncharacterized. Herein, we present a high-quality chromosome-scale genome for C. majus with a size of 1.06 Gb, and 91.21% of the sequences anchored onto 6 chromosomes, comprising 1,520 contigs with an N50 of 106.65 Mb. The genome is predicted to contain 25,203 protein-coding genes, with 98.2% have been functional annotated. The completeness of the genome is highlighted by a BUSCO score of 97.6%. This high-quality genome assembly provides a vital resource for future gene screening, drug discovery, and pharmacological exploration in C. majus.