Abstract
Food safety have received increasing attention in recent years, and rapid detection and thorough removal of organic contaminants is an important part of food safety control. In this work, a novel multi-functional photo-enzymatic nanoreactor HRP@Fe-NU-1003 is developed through the co-immobilization of horseradish peroxidase (HRP) and FeCl(2) on a photosensitive metal-organic frameworks (MOF) NU-1003. The bio-nanocluster can serve as an efficient biosensor in the detection of chloramphenicol (CAP), with a detection limit of 15.38 pg mL(-1), which is 62 times greater than that of the conventional HRP- enzyme-linked immunosorbent assay method. Besides its detecting capability, the nanoreactor also exhibits high efficiency in the photocatalytic degradation of CAP, which can remove 50 µg mL(-1) of CAP thoroughly within 30 min, and the mineralization efficiency of CAP reaches 61%. In this material, Fe-NU-1003 not only acts as a protecting shell to prevent HRP from deactivation, but improves detecting sensitivity and photocatalytic performance. Mechanism studies show that FeCl(2) enhances its photocatalytic performance through promoting electron (e(-))-hole (h(+)) separation and photocurrent transfer. More importantly, the heterogeneous material possesses high stability and can be recycled at least five rounds while its photocatalytic performance maintained at a high level. This strategy provides a new approach for the detection and degradation of pollutants.