Conclusion
Lipoic acid was found to be effective in reducing postoperative adhesion formation in a rat model. Amaç: Tam kat uterus hasarı yapılmış deneysel sıçan modelinde lipoik asitin postoperatif pelvik adezyonlar üzerindeki etkisinin incelenmesi amaçlanmıştır. Gereç ve yöntemler: Yirmi sekiz dişi Wistar albino sıçan dört gruba randomize edildi. Uterin hasar grubu kontrol, 15 gün ve 30 gün, uterin hasar + lipoik asit grubu 15 ve 30 gün. Tam kat uterin hasar her bir uterin boynuzdan 1.0 cm uzunlukta, mezometriyumu salim bırakarak insizyon yapılarak ve sütüre edilerek oluşturuldu. Adezyonların yaygınlığı ve şiddeti görsel skorlama sistemi ile ve immunohistokimyasal olarak yapıldı. Bulgular: Adezyon skorları grup 1, 2, 3 ve 4 için sırasıyla yaygınlık: 2.00±0.81, 2.14±0,69 0.71±0.75, 0.85±0.69 ve şiddet: 2.28±0.48, 2.14±0.69, 0.85±0.69,1.14±0.69 olarak belirlendi. Adezyon yaygınlık ve şiddeti lipoik asit ile tedavi edilen grupta anlamlı olarak daha azdı ancak kısa veya uzun süreli kullanım arasında fark yoktu. Hem Vitronectin hem u-Par boyanması tedavi grubunda kontrole göre anlamlı olarak artmıştı. Sonuç: Lipoik asit deneysel sıçan modelinde postoperatif adezyon oluşumunu önlemede etkili bulundu.
Material and methods
Twenty-eight female Wistar albino rats were randomised into four groups: uterine trauma control, 15 days and 30 days, and uterine trauma + lipoic acid, 15 days and 30 days. A full thickness defect was established by incising a segment of approximately 1.0 cm in length from each uterine horn, leaving the mesometrium intact. Extension and severity of the adhesions in each group were scored by a visual scoring system and evaluated immunohistochemically.
Methods
Twenty-eight female Wistar albino rats were randomised into four groups: uterine trauma control, 15 days and 30 days, and uterine trauma + lipoic acid, 15 days and 30 days. A full thickness defect was established by incising a segment of approximately 1.0 cm in length from each uterine horn, leaving the mesometrium intact. Extension and severity of the adhesions in each group were scored by a visual scoring system and evaluated immunohistochemically.
Objective
To investigate the effects of lipoic acid in the prevention of postoperative pelvic adhesions by a visual scoring system and immunohistochemistry in a rat uterine horn model with full thickness injury. Material and
Results
Adhesion scores were 2.00±0.81, 2.14±0.69 0.71±0.75, and 0.85±0.69 for extent and 2.28±0.48, 2.14±0.69, 0.85±0.69, and 1.14±0.69 for severity in Groups 1, 2, 3 and 4, respectively. Adhesion extent and severity were significantly less for groups treated by lipoic acid but no difference was observed between long and short administration. Both Vitronectin and u-PAR staining were significantly increased in treatment groups when compared to the control group.