Abstract
Mitochondria are cellular organelles that are the place of many metabolic processes and thus have a significant impact on the proper functioning of the organism. These organelles respond easily to environmental stimuli and cellular energy demands. To ensure the proper functioning of mitochondria, a high supply of specific nutrients is needed. Literature reports suggest that a favorable profile of the intestinal microbiota may improve the functioning of the mitochondria. The gut microbiota transmits a signal to the mitochondria of the mucosa cells. This signaling alters mitochondrial metabolism, activates cells of the immune system, and alters intestinal epithelial barrier functions. The aim of the study is to determine the relative number of mtDNA copies and to analyze the mitochondrial expression of genes related to respiratory chain proteins and energy metabolism in the intestinal mucosa and cecal tonsils of broiler chickens injected on the d 12 of egg incubation with various prebiotics. 300 incubated eggs of Ross 308 broiler chicken on d 12 of incubation were injected with: control group with physiological saline, prebiotics: XOS3, XOS4, MOS3, and MOS4. On d 42 after hatching, 8 individuals from each group were sacrificed. Cecal mucosa and cecal tonsils were collected postmortem for DNA and RNA isolation. Relative mitochondrial DNA copy number analysis was performed by qPCR method using 2 calculation methods. Gene expression analysis of the cecal tonsils and cecal mucosa was performed by RT-qPCR for the gene panel selected based on literature data and gene functions related to mitochondria: CS, EPX (MPO), CYCS, TFAM, NRF1, ND2, MnSOD (SOD2). As the results showed the overall mt DNA copy number is stable in both tissues. The significant change in gene expression in cecal mucosa was induced by XOS4 and MOS3. Both prebiotics caused upregulation of gene expression. In cecal tonsils all prebiotics caused downregulation of entire set of genes under the analysis. Statistically significant results of gene expression were detected for CYCS, ND2, NRF, TFAM for all experimental groups.