Quantitation of Dam methyltransferase in Escherichia coli

大肠杆菌中 Dam 甲基转移酶的定量分析

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Abstract

An antiserum against Escherichia coli Dam methyltransferase has been developed in rabbits and employed to detect and quantitate the enzyme in immunoblots. A wild-type, rapidly growing E. coli cell (doubling time = 30 min) was found to contain about 130 molecules of Dam methyltransferase.

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