Environmental toxicants perturb human Sertoli cell adhesive function via changes in F-actin organization mediated by actin regulatory proteins

环境毒物通过肌动蛋白调节蛋白介导的 F- 肌动蛋白组织变化干扰人类塞托利细胞粘附功能

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作者：Xiang Xiao, Dolores D Mruk, Elizabeth I Tang, Chris K C Wong, Will M Lee, Constance M John, Paul J Turek, Bruno Silvestrini, C Yan Cheng

期刊：

Human Reproduction

影响因子：

6.000

时间：

2014

起止号：

2014 Jun;29(6):1279-91.

doi：

10.1093/humrep/deu011

种属：

Human

研究方向：

细胞生物学

细胞类型：

其它细胞

Methods

Human Sertoli cells from three subjects were cultured in F12/DMEM containing 5% fetal bovine serum. Changes in protein expression were monitored by immunoblotting using specific antibodies. Immunofluorescence analyses were used to assess changes in the distribution of adhesion proteins, F-actin and actin regulatory proteins following exposure to two toxicants: cadmium chloride and bisphenol A (BPA). Main

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