Abstract
Burkholderia gladioli is a Gram-negative bacterium with multi-host adaptability as both a plant pathogen and an opportunistic human pathogen. It is also known as a producer of diverse bioactive metabolites. This dataset presents the first complete genome of B. gladioli isolated from Thailand, designated strain BGLCN, recovered from rice seedlings in Chai Nat Province. In preliminary assays, this strain exhibited in vitro antibacterial activity against human pathogens, including Acinetobacter baumannii, Escherichia coli, and Staphylococcus aureus, making it a promising candidate for the study of antibacterial compounds. The genome was sequenced using both Illumina NovaSeq and Oxford Nanopore PromethION platforms. A hybrid assembly generated two complete chromosomes and four plasmids with total genome size of 7.93 Mb with a GC content of 67.83 % and 100 % completeness. The genome contains 7266 coding sequences, 15 rRNA genes, and 84 tRNA genes. Phylogenomic analysis showed that BGLCN is closely related to rice-derived isolates from Vietnam. Biosynthetic gene cluster analysis using antiSMASH and DeepBGC revealed several putative secondary metabolite pathways, including polyketides, nonribosomal peptides, and saccharides. This dataset provides a valuable reference for comparative genomics, phylogenetic studies, and exploration of bioactive compound biosynthesis in B. gladioli. The genome is available under BioProject PRJNA1337644.