Abstract
Microbial culturomics represents a completely new approach to investigate microbial diversity by using different optimized culture conditions, mass spectrometry, genome sequencing and annotation and phenotypic description that allow for an extensive characterization of new species and the study of the human microbiome. Here we present four new species within the genus Paenibacillus: 'Paenibacillus bouchesdurhonensis' strain Marseille-P3071(T), 'Paenibacillus rubinfantis' strain MT18(T), 'Paenibacillus senegalimassiliensis' strain SIT18(T) and 'Paenibacillus tuaregi' strain Marseille-P2472(T), which are all facultatively aerobic and Gram-positive bacilli.