Abstract
We describe and illustrate three new species of Bertolonia, all endemic to the state of Espírito Santo, Brazil. Bertolonia duasbocaensis and B. macrocalyx occur close to each other, in the municipalities of Cariacica and Viana. Bertolonia ruschiana has a wider distribution, occurring in the municipalities of Santa Leopoldina, Santa Maria de Jetibá and Santa Teresa. The first two species are classified as critically endangered (CR), and the latter as endangered (EN). We also present an identification key for the species of Bertolonia that occur in Espírito Santo.